![]() ![]() These, in turn, will directly benefit the "yerba mate" industry, one of our most profitable economic activities. Conclusions The data presented herein constitutes a novel contribution, and a useful information platform that will enhance the generation of new "yerba mate" varieties, the improvement of the crop´s genetic background, and the devise of original transgenesis experiments. Thirty-five potential mononucleotidic and one dinucleotidic microsatellite loci were detected, their utility as markers remains to be evaluated. Fourty-nine repeats were identified, 27 palindromic and 22 forward. In total, 114 unique genes were detected 80 are coding sequences, 30 tRNAs and 4 rRNAs. It shows the typical quadripartite structure, having a LSC of 87,148bp two IRs of 26,076bp each, and a SSC of 18,310bp. A consensus sequence of 157.6bp was obtained for the complete plastome. Results As the sequencing result, 492,515bp were generated (in 56 contigs from 4 individuals). Plastidic microsatellite loci and the corresponding primer pairs were detected using the WebSat server. The number and location of repeats were assessed using REPuter. The reading frameworks were adjusted using sequences of Ilex cornuta as references, with the NCBI-BLAST algorithms and the MSWAT web server. Specific PCR primers were designed with Primer3Plus and Primer-BLAST, to check the junctions between the large (LSC) and small (SSC) single-copy segments and the two inverted repeats (IRs). A consensus sequence was obtained with Sequencher v4.1.4 (GeneCodes Corporation) the annotation was carried-out with CpGAVAS. We used bioinformatic tools to verify and assemble a definite plastome. There, a preliminary contig assembly was attempted. The samples were sequenced using 454 GSFLX+Roche at the INDEAR (Rosario, Santa Fe). The plastidic DNA was extracted adapting protocols. Materials and methods First, intact chloroplasts were isolated from fresh materials using the Chloroplast Isolation Kit (Sigma). The second column contains size of the sequence in bp. To contribute to its genetic knowledge, we faced the sequencing of the chloroplastgenome, analyzing its structure and gene content. new icons will appear once we assemble the sequences into contigs. Phytochemical studies on this species abound in the literature notably, the information about basic genetics is very limited. In Argentina the production of "yerba mate" is restricted to Misiones and Corrientes, due to the climate and soil requirements of the crop. Several medicinal properties are attributed to the high concentrations of various secondary metabolites, minerals and vitamins. The custom of drinking ?mate? is a legacy of the Guaraní culture strongly rooted in our society. Its economic value relies on the usage of the leaves and twigs, to prepare a popular infusion. Background The "yerba mate" tree (Ilex paraguariensis) is a perennial native to subtropical South America. Güiraldes 2620, Ciudad Universitaria, Pab. The plastome of the Yerba Mate tree Jimena Cascales1,2, Mariana Bracco1, Lidia Poggio1,2, Alexandra M Gottlieb1,2 1Laboratorio de Citogenética y Evolución, Departamento de Ecología, Genética y Evolución, IEGEBA (UBA-CONICET), FCEyN, UBA. ![]()
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